Two poplar methyl salicylate esterases display comparable biochemical properties but divergent expression patterns.
Identifieur interne : 003446 ( Main/Exploration ); précédent : 003445; suivant : 003447Two poplar methyl salicylate esterases display comparable biochemical properties but divergent expression patterns.
Auteurs : Nan Zhao [États-Unis] ; Ju Guan ; Farhad Forouhar ; Timothy J. Tschaplinski ; Zong-Ming Cheng ; Liang Tong ; Feng ChenSource :
- Phytochemistry [ 0031-9422 ] ; 2009.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires (MeSH), Esterases (génétique), Esterases (métabolisme), Modèles moléculaires (MeSH), Populus (enzymologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (physiologie), Spécificité du substrat (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- enzymologie : Populus.
- génétique : Esterases, Protéines végétales.
- métabolisme : Esterases, Protéines végétales.
- physiologie : Régulation de l'expression des gènes végétaux.
- Données de séquences moléculaires, Modèles moléculaires, Spécificité du substrat, Séquence d'acides aminés.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Esterases, Plant Proteins.
- chemical , metabolism : Esterases, Plant Proteins.
- enzymology : Populus.
- physiology : Gene Expression Regulation, Plant.
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data, Substrate Specificity.
Abstract
Two genes encoding proteins of 98% sequence identity that are highly homologous to tobacco methyl salicylate (MeSA) esterase (SABP2) were identified and cloned from poplar. Proteins encoded by these two genes displayed specific esterase activities towards MeSA to produce salicylic acid, and are named PtSABP2-1 and PtSABP2-2, respectively. Recombinant PtSABP2-1 and PtSABP2-2 exhibited apparent Km values of 68.2+/-3.8microM and 24.6+/-1microM with MeSA, respectively. Structural modeling using the three-dimensional structure of tobacco SABP2 as a template indicated that the active sites of PtSABP2-1 and PtSABP2-2 were highly similar to that of tobacco SABP2. Under normal growing conditions, PtSABP2-1 showed the highest level of expression in leaves and PtSABP2-2 was most highly expressed in roots. In leaf tissues of poplar plants under stress conditions, the expression of PtSABP2-1 was significantly down-regulated by two stress factors, whereas the expression of PtSABP2-2 was significantly up-regulated by four stress factors. The plausible mechanisms leading to these two highly homologous MeSA esterase genes involved in divergent biological processes in poplar are discussed.
DOI: 10.1016/j.phytochem.2008.11.014
PubMed: 19136124
Affiliations:
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Tschaplinski</name></author><author><name sortKey="Cheng, Zong Ming" sort="Cheng, Zong Ming" uniqKey="Cheng Z" first="Zong-Ming" last="Cheng">Zong-Ming Cheng</name></author><author><name sortKey="Tong, Liang" sort="Tong, Liang" uniqKey="Tong L" first="Liang" last="Tong">Liang Tong</name></author><author><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19136124</idno><idno type="pmid">19136124</idno><idno type="doi">10.1016/j.phytochem.2008.11.014</idno><idno type="wicri:Area/Main/Corpus">003690</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003690</idno><idno type="wicri:Area/Main/Curation">003690</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003690</idno><idno type="wicri:Area/Main/Exploration">003690</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Two poplar methyl salicylate esterases display comparable biochemical properties but divergent expression patterns.</title><author><name sortKey="Zhao, Nan" sort="Zhao, Nan" uniqKey="Zhao N" first="Nan" last="Zhao">Nan Zhao</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Sciences, University of Tennessee, 252 Ellington Plant Science Bldg., 2431 Joe Johnson Drive, Knoxville, TN 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, University of Tennessee, 252 Ellington Plant Science Bldg., 2431 Joe Johnson Drive, Knoxville, TN 37996</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Guan, Ju" sort="Guan, Ju" uniqKey="Guan J" first="Ju" last="Guan">Ju Guan</name></author><author><name sortKey="Forouhar, Farhad" sort="Forouhar, Farhad" uniqKey="Forouhar F" first="Farhad" last="Forouhar">Farhad Forouhar</name></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name></author><author><name sortKey="Cheng, Zong Ming" sort="Cheng, Zong Ming" uniqKey="Cheng Z" first="Zong-Ming" last="Cheng">Zong-Ming Cheng</name></author><author><name sortKey="Tong, Liang" sort="Tong, Liang" uniqKey="Tong L" first="Liang" last="Tong">Liang Tong</name></author><author><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="ISSN">0031-9422</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Esterases (genetics)</term><term>Esterases (metabolism)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Models, Molecular (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (enzymology)</term><term>Substrate Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Données de séquences moléculaires (MeSH)</term><term>Esterases (génétique)</term><term>Esterases (métabolisme)</term><term>Modèles moléculaires (MeSH)</term><term>Populus (enzymologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term><term>Spécificité du substrat (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Esterases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Esterases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Esterases</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Esterases</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Modèles moléculaires</term><term>Spécificité du substrat</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two genes encoding proteins of 98% sequence identity that are highly homologous to tobacco methyl salicylate (MeSA) esterase (SABP2) were identified and cloned from poplar. Proteins encoded by these two genes displayed specific esterase activities towards MeSA to produce salicylic acid, and are named PtSABP2-1 and PtSABP2-2, respectively. Recombinant PtSABP2-1 and PtSABP2-2 exhibited apparent Km values of 68.2+/-3.8microM and 24.6+/-1microM with MeSA, respectively. Structural modeling using the three-dimensional structure of tobacco SABP2 as a template indicated that the active sites of PtSABP2-1 and PtSABP2-2 were highly similar to that of tobacco SABP2. Under normal growing conditions, PtSABP2-1 showed the highest level of expression in leaves and PtSABP2-2 was most highly expressed in roots. In leaf tissues of poplar plants under stress conditions, the expression of PtSABP2-1 was significantly down-regulated by two stress factors, whereas the expression of PtSABP2-2 was significantly up-regulated by four stress factors. The plausible mechanisms leading to these two highly homologous MeSA esterase genes involved in divergent biological processes in poplar are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19136124</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2009</Year><Month>02</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Two poplar methyl salicylate esterases display comparable biochemical properties but divergent expression patterns.</ArticleTitle><Pagination><MedlinePgn>32-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2008.11.014</ELocationID><Abstract><AbstractText>Two genes encoding proteins of 98% sequence identity that are highly homologous to tobacco methyl salicylate (MeSA) esterase (SABP2) were identified and cloned from poplar. Proteins encoded by these two genes displayed specific esterase activities towards MeSA to produce salicylic acid, and are named PtSABP2-1 and PtSABP2-2, respectively. Recombinant PtSABP2-1 and PtSABP2-2 exhibited apparent Km values of 68.2+/-3.8microM and 24.6+/-1microM with MeSA, respectively. Structural modeling using the three-dimensional structure of tobacco SABP2 as a template indicated that the active sites of PtSABP2-1 and PtSABP2-2 were highly similar to that of tobacco SABP2. Under normal growing conditions, PtSABP2-1 showed the highest level of expression in leaves and PtSABP2-2 was most highly expressed in roots. In leaf tissues of poplar plants under stress conditions, the expression of PtSABP2-1 was significantly down-regulated by two stress factors, whereas the expression of PtSABP2-2 was significantly up-regulated by four stress factors. The plausible mechanisms leading to these two highly homologous MeSA esterase genes involved in divergent biological processes in poplar are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Nan</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, University of Tennessee, 252 Ellington Plant Science Bldg., 2431 Joe Johnson Drive, Knoxville, TN 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guan</LastName><ForeName>Ju</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Forouhar</LastName><ForeName>Farhad</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Zong-Ming</ForeName><Initials>ZM</Initials></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Liang</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Feng</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>01</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="D004950">Esterases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004950" MajorTopicYN="N">Esterases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>06</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>11</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19136124</ArticleId><ArticleId IdType="pii">S0031-9422(08)00589-X</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2008.11.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name><name sortKey="Cheng, Zong Ming" sort="Cheng, Zong Ming" uniqKey="Cheng Z" first="Zong-Ming" last="Cheng">Zong-Ming Cheng</name><name sortKey="Forouhar, Farhad" sort="Forouhar, Farhad" uniqKey="Forouhar F" first="Farhad" last="Forouhar">Farhad Forouhar</name><name sortKey="Guan, Ju" sort="Guan, Ju" uniqKey="Guan J" first="Ju" last="Guan">Ju Guan</name><name sortKey="Tong, Liang" sort="Tong, Liang" uniqKey="Tong L" first="Liang" last="Tong">Liang Tong</name><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Zhao, Nan" sort="Zhao, Nan" uniqKey="Zhao N" first="Nan" last="Zhao">Nan Zhao</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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